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Sartorius Stedim Biotech introduces Sartobind® Cassettes

New modular membrane chromatography  solution for large-scale applications

Sartorius Stedim Biotech (SSB), a leading international supplier for  the biopharmaceutical industry,  has expanded its range of single-use membrane chromatography solutions with Sartobind® Cassettes. This convenient, pod-like modular system has been developed for commercial applications  in both  capture and polishing.

New Sartobind® cassettes offer the same flow path, bed heights (4 and 8 mm) and void volume ratios as Sartobind® capsules, and are compatible with Q, S, STIC PA and phenyl ligands. The new design goes beyond the previous 5  L size limitation for capsule formats,  expanding the boundaries of membrane chromatography. Multiple cassettes, each with 0.8 L or 1.6 L membrane volume, can be set up in three different stainless steel holders resulting in maximum membrane volumes of 20, 50 or 100 L, respectively. Data demonstrates direct scalability from the 3 mL Sartobind® nano capsule to 20.8 L (13 cassettes) in the  Pilot Filter Holder. Pressure-flow performance and the shape of breakthrough curves are identical to the smaller capsule sizes, independent of the number of cassettes used. Set up can be accomplished within minutes, even at manufacturing scale, whether for capture applications or for flow-through  removal of process contaminants.

The newly developed Sartobind® cassette system is a unique single-use solution enabling large-scale bind and elute membrane chromatography.

 

The capture  of large proteins such as viruses and virus-like particles  (VLPs), protein conjugates and  blood factors plays a key role in  modern bioprocessing. The unique Sartobind® cassette system now enables large-scale  bind-and-elute membrane chromatography for such targets. »For the first time, the high binding  capacity of membrane adsorbers  can be used at almost any scale  in  commercial vaccine manufacture.   Now, a 200 L Q anion exchange  column for adenovirus capture  can be easily replaced by a 20 L Q adsorber cassette system”, says Dr. Stefan Fischer-Frühholz, membrane chromatography expert at Sartorius Stedim Biotech.

New Sartobind® cassettes go beyond the previous size limits of membrane adsorbers and can be directly scaled from a 3 mL capsule (Nano) up to 20.8 L.

 

www.sartorius-stedim.com